Bio-efficacy of Pseudomonas fluorescens (7% WP and 5% SC formulations) against bacterial wilt disease of chili

Amitava Basu


Bacterial wilt caused by Ralstonia solanacearum is an important disease of chili crop in West Bengal. P.  fluorescens was employed to manage R. solanecearum under field condition. The combined use of seed and soil treatment were most effective (0.83 to 10.82% PDI) than sole use of seed or soil treatment (3.33 to 24.98% PDI). Vegetative growth and yield of chili were also influenced through integration of seed and soil treatments with P. fluorescens 7% WP @ 10 g/kg seed + 2.5 kg/ha as well as with P. fluorescens 5% SC applied @ 10 ml/kg seed + 2.5 lit/ha . The seed treatment when followed by soil treatment showed best result in respect of fresh root weight (6.63-7.43 g), dry root weight (2.53-2.80 g), fresh shoot  weight (55.45-60.23 g), dry shoot weight (24.25-26.33 g), plant height (44.83-47.53 cm) and yield (257.18-265.60 kg/ha) than  control. There was no adverse effect of P. fluorescens 7% WP and P. fluorescens 5% SC on soil beneficial microbes. The products increased the root and shoot weight, dry weight of seedlings and seedling vigor index than control. The per cent root colonization in sterilized and non sterilized soil was in the range of 80-89%.It may be concluded that, the combined application of P. fluorescens 7% WP as seed treatment @ 10 g/kg seed + soil treatment @ 2.5 Kg/ha or P. fluorescens 5% SC as seed treatment @ 10 ml/kg seed + soil treatment @ 2.5 lit/ha is suggested for effective management of bacterial wilt of chili.


Keywords— Chili, wilt, Ralstonia solanecearum, Pseudomonas flurorescens, biocontrol.



Anonymous, Seasonal report in chilli. Society, vol. 57, pp. 25-39, 2007.

C. Chattopadhyay and B. Sen, “Integrated management of fusarium wilt of muskmelon caused by Fusarium oxysporum.” Indian J. Myco. Pl. Pathol., vol. 26, pp. 162-170, 1996.

CIB&RC,Guidelines for registration of antagonistic bacteria under section 9(3B)&9(3)of the Insecticide act,1968, Govt. of India, 2011.

K. A. Gomez and A. A. Gomez, Statistical procedure for agriculture research, 2nd edition. Jhon wiley and sons, inc. London, UK, pp. 13-175, 1984.

International Seed Testing Association, “International rules for seed testing. Rules 1985.” Seed Sai. & TeahnoL., vol. 13, pp. 299-355, and Annexes 1985. Seed Sai. &TeahnoL., vol. 13, pp. 356-513, 1985.

S. K. Jayalakshmi, S. Raju, S. Usha Rani, V. I. Benagi and K. Sreeramulu, “Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. ciceri., Australian Journal of Crop Science Southern Cross Journals, vol. 3, pp. 44-52, 2009.

Y. D. Jing, Z. L. He and X. E. Yang, “Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils.” J. Zhejiang Univ. Sci., vol. 8, pp. 192–207, 2007.

John Davison, “Plant beneficial bacteria.” Bio-Technology, vol. 6, pp. 282-286, 1988.

Karuna Vishunavat and S. J. Kolte, Essentials of Phytopathological Techniques. Kalyani Publishers, New Delhi, p. 210, 2005.

J. W. Kloepper, S. Tuzun, L. Liu and G. Wei, “Plant growth-promoting rhizobacteria as inducers of systemic disease resistance.” In: R. D. Lumsden, J. L. Waughn Eds. Pest management: biologically based technologies. American Chemical Society Books, Washington, DC, pp. 156-165, 1993.

P. V. Minorsky, “On the inside”. Plant Physiol., vol. 146, pp. 323–324, 2008.

A. Muthukumar, “Management of chilli damping-offcaused by Pythium aphanidermatum (Edson) Fitz. with bacterial endophytes (Pseudomonas fluorescens) in glasshouse conditions.” Advances in Plant Sciences, vol. 21, pp. 295-298, 2008.

R. Ramesh, A. A. Joshi and M. P. Ghanekar, “Pseudomonas: Major endophytic bacteria to suppress bacterial wilt pathogen Ralstonia solanacearum in the egg plant (Solanum melongena L.).” World Journal of Microbiology and Biotechnology, vol. 25, pp. 47-55, 2009.

R. Rangeshwaran, J. Raj, and P. Sreeramakumar, “Identification of endophytic bacteria in chickpea (Cicer arietinum L.) and their effect on plant growth.” Journal of Biological Control, vol. 22, pp. 13-23, 2008.

D. Saravanakumar and R. Samiyappan, “ACC deaminase from Pseudomonas fluorescens mediated saline resistance in groundnut (Arachis hypogea) plants.” J Appl Microbiol, vol. 102, pp. 1283–1292, 2007.

A. V. Sturz and B. R. Christie, “Endophytic bacterial system governing red clover growth and development.” Annals of Applied Biology, vol. 126, pp. 285-290, 1995.

B. E. J. Wheeler, An Introduction to Plant Diseases. John Wiley and Sons Limited, London, p. 254, 1969.

Full Text: PDF


  • There are currently no refbacks.

Publisher by Asia Pacific Network for Sustainable Agriculture, Food and Energy Network (SAFE Network).